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The 2 protein bands from 40 kDa to thirty kDa were absent immediately after 24 h incubation at 45 °C. For the remainder of the bands, different concentrations of all three proteases retained protein bands corresponding to their initial molar masses (Figure 6a,b). The existence of bands at low molecular pounds in fish roe right before hydrolysis was unanticipated. While in the report by Ghelichi et al. [eight], slight peaks of a relatively very low molecular excess weight array (3–14 kDa) were being observed in defatted carp roe homogenate, perhaps as a result of an autolytic process taking place in fish roe and/or the defatting system bringing about the development of minimal-molecular-fat peptides.
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